application of citrate as a tricarboxylic acid (tca) cycle intermediate, prevents diabetic-induced heart damages in mice
نویسندگان
چکیده
objective(s):higher cellular reactive oxygen species (ros) levels is important in reducing cellular energy charge (ec) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. however, the relation between energy production and heart function is unclear. materials and methods:streptozotocin (stz, 150 mg/kg body weight) was injected intraperitoneally once to mice that had been fasted overnight for induction of diabetes. after diabetic induction, mice received citrate (5 µg/kg) through intraperitoneal injection every other day for 5 weeks. the caspase-3, plasminogen activator inhibitor 1 (pai1), protein kinase b (pkb), commonly known as akt and phosphorylated-akt (p-akt) proteins were examined to elucidate inflammation and apoptosis in the heart. for histological analysis, heart samples were fixed with 10% formalin and stained with hematoxylin-eosin (he) and sirius red to assess pathological changes and fibrosis. the expression levels[aga1] of marker proteins, tyrosine nitration, activity of atp synthase and succinyl-coa:3-ketoacid coenzyme a transferase-1 (scot), and ec were measured. results:intraperitoneal injection of citrate significantly reduced caspase-3 and pai-1 protein levels and increased p-akt level on the 5th week; ec in the heart was found to be increased as well. further, the expression level, activity, and tyrosine nitration of atp synthase and scot were not affected after induction of diabetes. conclusion: results indicate that application of citrate, a tricarboxylic acid (tca) cycle intermediate, might alleviate cardiac dysfunction by reducing cardiac inflammation, apoptosis, and increasing cardiac ec.
منابع مشابه
Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice
Objective(s):Higher cellular reactive oxygen species (ROS) levels is important in reducing cellular energy charge (EC) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. However, the relation between energy production and heart function is unclear. Materials and Methods:Streptozotocin (STZ, 150 mg...
متن کاملApplication of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice
OBJECTIVES Higher cellular reactive oxygen species (ROS) levels is important in reducing cellular energy charge (EC) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. However, the relation between energy production and heart function is unclear. MATERIALS AND METHODS Streptozotocin (STZ, 150 mg/...
متن کاملGenetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes.
The eight enzymes of the tricarboxylic acid (TCA) cycle are encoded by at least 15 different nuclear genes in Saccharomyces cerevisiae. We have constructed a set of yeast strains defective in these genes as part of a comprehensive analysis of the interactions among the TCA cycle proteins. The 15 major TCA cycle genes can be sorted into five phenotypic categories on the basis of their growth on ...
متن کاملTricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise.
We examined the relationship between tricarboxylic acid (TCA) cycle intermediate (TCAI) pool size, TCA cycle flux (calculated from leg O2uptake), and pyruvate dehydrogenase activity (PDHa) in human skeletal muscle. Six males performed moderate leg extensor exercise for 10 min, followed immediately by intense exercise until exhaustion (3.8 ± 0.5 min). The sum of seven measured TCAI (ΣTCAI) incre...
متن کاملThe Tricarboxylic Acid Cycle in Dictyostelium discoideum
Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۱، صفحات ۴۳-۴۸
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023